Hence, the general reaction is: The macrolide antibiotics contain a large macrocyclic lactone ring to which one or more deoxysugar may be attached.
These antibiotics inhibit bacterial protein biosynthesis by binding reversibly to the subunit 50S of the bacterial ribosome and preventing translocation of peptidyl-t RNA.
Aliquots (50 μl) were analyzed directly by LC-MS-MS.
In all experiments, the electrospray capillary voltage was held at 3.1 k V, and the cone voltage was at 30 V.
Source block and drying gas temperatures were maintained at 110 and 330°, respectively.
2-Keto-3-deoxy-D-gluconic acid is soluble (in water) and a moderately acidic compound (based on its p Ka).
Within the cell, 2-keto-3-deoxy-D-gluconic acid is primarily located in the cytoplasm.